Fig. 2. Effects of non-selective muscarinic and nicotinic acetylcholine receptor antagonists on EA-induced anti-allodynia.

Rats with cold allodynia induced by a single injection of oxaliplatin were randomly divided into three groups. The behavioral tests for cold allodynia were performed before an intraperitoneal injection of antagonists and after EA treatment: (A) NS (n=5), (B) atropine (n=5), and (C) mecamylamine (n=7). The analgesic effect of EA at ST36 was blocked by intraperitoneal injection of atropine (non-selective muscarinic receptor antagonist, 1 mg/kg), but not by intraperitoneal injection of mecamylamine (non-selective nicotinic receptor antagonist, 2 mg/kg). Data are presented as mean±S.E.M.; ^**p<0.01; by paired t-test.