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. 2016 Jul 4;10:132. doi: 10.3389/fnbeh.2016.00132

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Copyright © 2016 Genné-Bacon, Trinko and DiLeone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ΔFosB levels are increased in the medial hypothalamus (mHyp) following repeated mT exposure. Western blots for FosB and β-actin (loading control) were carried out on fresh-frozen brain tissue dissected from the mHyp (A). Protein bands were distinguished by size (B,C). Band intensities were calculated using ImageJ gel analysis tools. ΔFosB protein levels were significantly elevated (D) in the mHyp following 6 weeks of daily mT exposure compared to BA-exposed mice. Neither FosB levels (E), nor β-actin (F) differed between scent groups. All error bars shown represent SEM.