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. 2016 Jun 20;2016:1464725. doi: 10.1155/2016/1464725

Figure 5.

Figure 5

(a) The H&E staining and Masson's trichrome staining showed that more compact structure and more collagen deposition were found in ASC treated group compared with PBS treated group at day 14. This finding was also verified by exploring the distribution of collagen I and collagen III through picrosirius red staining. The ratio of collagen I and collagen III was much higher in ASCs treated group than that of PBS treated group. Scale bar 200 μm. (b) Immunohistochemistry with TGF-β3 in both epidermis-dermis of the skin graft wound margin and graft bed-facial area. The TGF-β3 level was significantly higher than those in PBS group. Scale bar 50 μm. (c) Quantitative evaluation of ratio of collagen types I and III. (d and e) Quantitative evaluation of TGF-β3 expression of the skin graft wound margin and graft bed-facial area, respectively. Data are presented as the mean ± SD of three separate experiments. ∗Compared with PBS, p < 0.05.