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. 2016 Aug;22(8):1146–1152. doi: 10.1261/rna.056796.116

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© 2016 Wu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Ψ93 formation induced by rapamycin treatment. Yeast cells were treated with DMSO alone (lanes 1 and 2), rapamycin dissolved in DMSO (lanes 3 and 4), or tunicamycin dissolved in DMSO (lanes 5 and 6) for ∼12 h, and then harvested. Pseudouridylation assay was then performed. At the moment of cell harvest, DMSO-treated cells reached an OD₆₀₀ of ∼10, whereas rapamycin- and tunicamycin-treated cells reached an OD₆₀₀ of only ∼2–3.