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. 2016 Aug;22(8):1146–1152. doi: 10.1261/rna.056796.116

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© 2016 Wu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — The RAS/cAMP signaling pathway is not involved in the regulation of Ψ93 formation. Yeast strain (ras1Δ GAL-RAS2), where the chromosomal RAS1 was deleted and the chromosomal RAS2 promoter was replaced by a GAL promoter, was cultured in exactly the same way as in Figure 4A. Lanes 1 and 2, cells grown in galactose medium to OD 1.5; lanes 3 and 4, cells grown in glucose medium to OD 1.0 after transferring some cells from galactose medium to glucose medium; lanes 5 and 6, cells grown in glucose medium to OD 1.5 after transferring some cells from galactose medium to glucose medium; lanes 7 and 8, cells grown in galactose medium to saturation (OD 15). Upon completion of cell culture, total RNA was isolated and pseudouridylation assay performed. Signals representing constitutively formed pseudouridines (Ψ35, Ψ42, and Ψ44) and inducibly formed Ψ93 are indicated.