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. 2016 Aug;22(8):1190–1199. doi: 10.1261/rna.056242.116

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© 2016 Lopes et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Effects of TbTrl1 RNAi on the growth rate of procyclic form T. brucei cells. (A) Cloned procyclic trypanosome cells harboring the TbTrl1 RNAi plasmid construct were incubated in culture medium without (−tet, black circles) or with (+tet, black squares) tetracycline. The rate of cell growth was monitored daily, and the cell numbers were plotted in a logarithmic scale as cumulative cell numbers. (B) tbtrl1 intracellular mRNA levels monitored by qPCR from the corresponding RNAi experiments after 2, 4, and 7 d of induction. The expression levels were normalized using α-tubulin as an endogenous control, P < 0.05 (n = 3).