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. 2016 Aug;22(8):1239–1249. doi: 10.1261/rna.056077.116

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© 2016 Chung et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — RNA substrate of Gld2. Gld2 was incubated with different RNA substrates and [α-P³²]-ATP as indicated. Formation of α-³²P labeled RNA products was monitored by electrophoretic separation and subsequent phosphorimaging. Gld2 catalyzed [α-³²P]-ATP addition to RNA substrates pre-let-7a (72 nt), mature human miRNAs let-7a-5p (22 nt), miR122 (22 nt), an oligo(A) tail mimic 15A (15 nt), and total E. coli tRNA, and total human mRNA. C is no enzyme control.