AIFA-based scheme in detecting ALK-positive NSCLC using an integrated approach with IHC and FISH. All antibody clones against ALK are accepted employing a scoring system, then requiring FISH confirmation in indeterminate cases at IHC (scores 1+ and 2+). AIFA, Agenzia Italiana del Farmaco; ALK, anaplastic lymphoma kinase; NSCLC, non-small cell lung cancer; IHC, immunohistochemistry; FISH, fluorescence in situ hybridization.