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. 2016 Jun 15;8(6):2681–2692.

Figure 5.

Figure 5

Notch-1 overexpression on NF-κB DNA-binding activity and its target genes. The SKBR-3 cells were transfected with human Notch-1 cDNA for 36 hs.The NF-κB DNA-binding activity was detedted by EMSA (A). The expression of IL-8, MMP-9 and VEGF was detected by western blot assay (B) and RT-PCR assay (C). The culture supernatant of IL-8, MMP-9 and VEGF was detected by ELISA (D-F). Statistical significance of three or more experiments was determined by performing a two-tailed, unpaired Student’s t-test for two comparisons. vs control, *p < 0.05.