Figure 3.

HDAC7 modulates Bcl6 gene transcription in B cells. B cells were separated from the mouse spleen and were stimulated with flagellin (FGN; 5 μg/ml) in the culture for 3 h and analyzed by ChIP assay. The bar graphs indicate the levels of pHDAC7 (A), H3K9me (B), H3K27me (C), Pol II (D) and STAT3 (E) at the Bcl6 promoter locus. (F) The immune blots indicate the results of HDAC7 gene knockdown. (G and H) Cells were treated with IL-6 (200 ng/ml) and FGN (5 μg/ml) for 48 h; the cells were analyzed by RT-qPCR and Western blotting. The bars indicate the mRNA level of Bcl6 in the B cells (G); the Western blots indicate the protein level of Bcl6 (H). FGNa and FGNb: Control shRNA (a) and HDAC7 shRNA (b) treated B cells were treated with FGN in the culture. The data of bars are presented as mean ± SD. *, p<0.01, compared with the saline group. The data are representatives of 3 independent experiments.