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. 2016 Jun 15;8(6):2748–2757.

Figure 4.

Figure 4

Flagellin facilitates IgE expression in B cells. (A and B) The Western blots indicate the RNAi results of Bcl6 (A; a) and TLR5 (B; b). (C and D) Naive B cells were isolated from the mouse spleen by MACS. The cells were treated in the culture as denoted on the X axis of panel C for 96 h. The bars indicate the levels of mRNA (C), the immune blots indicate the protein (D) of IgE in B cells. (E) A sketch of an IgE promoter luciferase reporter gene. (F) The bars indicate the IgE promoter activities. The label “c” indicates the cells were treated with control shRNA. (G-I) Naive B cells were treated in the culture for 48 h as denoted on the X axis. The cells were analyzed by ChIP assay. The bars indicate the levels of p300 (G), H3K4ac (H), H3K9ac (I) and STAT6 (J) at the IgE promoter locus. #, the presence of garcinol (15 μM). The data of bars are presented as mean ± SD. *, p<0.01, compared with the medium group. The data are a representative of 3 independent experiments.