Figure 2.

Suppressing miR-322 rescued cardiomyocyte apoptosis under hypoxia condition. A. Cardiomyocytes were transduced with a lentivirus containing mmu-miR-322 inhibitor (L-miR322-Inhibitor), or a lentivirus containing negative control scrambled miRNA (L-C-miR). After transduction was stabilized, qRT-PCR was carried out to evaluate the efficiency of miR-322 downregulation in cardiomyocyte (*P < 0.05). B. Lentiviral-transduced cardiomyocytes were then treated with hypoxia condition for 2 h. 24 h after that, a TUNEL assay was carried out and immune-fluorescent images were shown for cardiomyocyte apoptosis (Red: TUNEL; Blue: DAPI). C. The percentages of apoptotic cardiomyocytes under hypoxia condition were compared between cells transduced with L-C-miR and cells transduced with L-miR322-Inhibitor (*P < 0.05).