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. 2016 Jun 15;8(6):2812–2819.

Figure 3.

Figure 3

BDNF is the target gene of miR-322 in cardiomyocyte under hypoxia condition. A. 3’-UTR of BDNF gene is predicted to be bound by miR-322 (top panel). Alternatively, a mutated BDNF 3’-UTR was created to dissociate miR-322 binding (bottom panel). B. HEK293T cells were co-transfected with mmu-miR-322 mimics, and a Control luciferase reporter, a luciferase reporter expressing mutated (m) BDNF 3’-UTR, or a luciferase reporter expressing BDNF 3’-UTR. Three groups of HEK293T cells were then underwent a dual-luciferase reporter assay to measure the relative luciferase activities (*P < 0.05). C. QRT-PCR was carried out to compare the expression levels of BDNF in normally cultured cardiomyocytes (Control), cardiomyocytes under hypoxia condition (Hypoxia), L-C-miR transduced cardiomyocytes under hypoxia condition (L-C-miR + hypoxia), and L-miR322-Inhibitor transduced cardiomyocytes under hypoxia condition (L-miR322-Inhibitor + hypoxia) (*P < 0.05, ∆P > 0.05).