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. 2016 May 27;35(13):1385–1399. doi: 10.15252/embj.201593458

Figure 2. HSV‐1 does not replicate productively in macrophages, but ICP27 expression correlates inversely with type I IFN production.

Figure 2

  1. MDMs were infected for 18 h with HSV‐1 (KOS, MOI 1). Cytosolic viral proteins were detected by mass spectrometry using iTRAQ labeling. Identified viral proteins are shown.
  2. MDMs were infected with the KOS or F strains of HSV‐1 (MOI 3). Cell lysates were harvested 12 hpi, and levels of ICP27 and β‐actin were determined by Western blotting.
  3. HFFs and MDMs were infected with HSV‐1 (KOS, MOI 0.1, and 1.0). Culture medium was replaced 3 hpi and isolated 24 hpi for viral plaque assay. Data are presented as means of triplicates ± SD.
  4. THP1 cells were infected with the indicated strains of HSV‐1 (MOI 3) for 12 h. Cell lysates were isolated, and levels of ICP27 and β‐actin were determined by Western blotting.

Source data are available online for this figure.