Figure 6. There is no infiltration of blood monocytes in the dorsal horn after SNT.

(a) Representative blood cytometry data from CX₃CR1^CreER/+/R26^tdTomato/+ reporter mice showing the CD11b⁺/CX₃CR1⁺ population at 3 days and 3 weeks post tamoxifen administration (n=3 for each time point). (b) Iba1 staining (green) showing all spinal dorsal horn microglia were tdTomato (red) positive at POD3 after SNT in CX₃CR1^CreER/+/R26^tdTomato/+ mice with 3-week interval between TM and DT treatment. The results indicate no peripheral monocyte infiltration to spinal cord at POD3 after SNT. Higher-magnification images from boxed regions in larger images showing microglial morphology (n=3 mice for each group, n=3 images for each animal). Scale bar, 100 μm.