CTGF mRNA expression levels in skin (dSSc) and lung (LSSc) fibroblasts isolated from scleroderma patients and treated with the IRE1α inhibitor 4μ8C. *P = 0.018 for dSSc (n = 6) and P = 0.003 for LSSc (n = 3).
Collagen 1α2 mRNA expression levels in the same samples as in (A). *P = 0.0030 for dSSc and P = 0.0036 for LSSc.
Quantification of collagen protein levels in the same samples as in (A). *P = 0.010.
Immunohistochemical staining to detect BiP protein in fibroblasts isolated from scleroderma patients treated or not with 4μ8C. Scale bars = 20 μm.
mRNA levels of αSMA in primary lung fibroblasts isolated from patients with either cystic fibrosis (CF), chronic obstructive pulmonary disease (COPD), or asthma. *P = 0.00001 for asthma versus healthy controls, and P = 0.00003 for ctrl versus 4μ8C in asthma patients.
Quantification of mRNA levels for BiP in same samples as in (E). *P = 0.0023 between asthma and healthy controls and P = 0.0031 between 4μ8C‐treated and control treatment in fibroblasts from asthma patients.
Data information: Statistical significance was evaluated using Student's
t‐test. Significant differences are indicated with * and exact
P‐values given. Error bars indicate s.e.m.
n = 3 in (E, F).
