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. 2016 May 19;8(7):761–778. doi: 10.15252/emmm.201506059

Figure 1. CLU is required for proliferation and cycling of PC3 cells.

Figure 1

  1. Cell proliferation for PC3 cells transfected with siSCR or siCLU monitored in real time for 60 h with SP Dynamic xCELLigence system (Voltage 2.5 K). Error bars represent mean ± SEM, n = 4. Slopes compared by ANCOVA test, P < 0.0001.
  2. Left panel: Cell cycle profile of PC3 cells transfected with siSCR or siCLU. Right panel: Percentage quantification of variation in G1, S, and G2/M phases. Error bars represent mean ± SEM, n = 3, **P < 0.01 by paired Student's t‐test (G2/M population P = 0.0058; G1 P = 0.008). Inset: Western blot with CLU antibody in PC3 cells after CLU silencing. Vinculin was used as loading control.
  3. Left panel: RNA microarray analysis comparing PC3 cells transfected with siCLU versus siSCR, and Kinexus phosphokinome microarray analysis comparing LNCaP cells transfected with siCLU versus siSCR. Target genes expression is shown as fold change relative to siSCR, P ≤ 0.05 by an unpaired t‐test for all the genes listed. Central panel: Venn diagram to graphically illustrate the overlap of mRNA and proteins involved in the regulation of mitosis in both transcriptome and phosphokinome analysis. The comparison of these two experiments shows modification of 4 mitosis regulators. Right panel: Western blot validating the expression levels of target proteins.