FIG. 5.

Caspase-9 and −3 activation in FECDi and MN-induced normal cells. HCEnC-21T and HCECi cells were exposed to 50 μM MN in a time-dependent manner. FECDi cells were untreated or in the basal state. Cells were collected and blotted for cleaved caspase-9 and cleaved caspase-3. Procaspase -9, total caspase-3, and β-actin were blotted for normalization and loading control. Bands represent (A) pro- and cleaved caspase-9 (top) and pro- and cleaved caspase-3 (bottom) in HCEnC-21T cells; (B) pro- and cleaved caspase-9 (top) and total and cleaved caspase-3 (bottom) in HCECi cells. Untreated FECDi cells display cleaved caspase-9 and caspase-3 activation. (C) Quantification of representative blots (n = 3). Data are expressed as the mean ± SE. Student's t-test was applied for statistical significance. *p < 0.05 from control (0) or versus normal cell line.