Figure 3. Reducing PSAP levels increases PGRN levels.

(a) PGRN levels in the media and cell lysates of HeLa cells were measured by the R&D ELISA post siRNA transfection with either a control siRNA (white bars) or PSAP-targeted siRNAs #1 and #2 (black and grey bars, respectively (n≥12 per group). (b) Immunoblot of media and lysates from HeLa cells transfected with control or PSAP siRNAs. (c) Quantification of PGRN immunoreactivity from immunoblots of HeLa cell media and lysates post PSAP knockdown with PSAP siRNA #1 (black bars), #2 (grey bars) or a control siRNA (white bars; n=12 per group). (d) GRN mRNA levels were quantified in HeLa cells post PSAP knockdown (n=5 per group). In all graphs, the values represent the mean±s.e.m. *Differs from controls, P<0.001 by one-way ANOVA.