Figure 7. Increasing PSAP levels results in changes in PGRN oligomerization.

(a,b) R&D (a) or Adipogen (b) PGRN ELISA measurements in HeLa media from control- (white bars) or PSAP-transfected (black bars) cells after a 3-h incubation with or without guanding-HCl (n=3 per group). (c) Native gel of media from HeLa cells transfected with either a control PSAP overexpression plasmid. (d) Immunoblot of media from HeLa cells transfected with either a control or PSAP plasmid that had been treated with (+) or without (−) 1 mM BS³. (e) Native gel of conditioned media obtained from HeLa cells transfected with a control or PSAP siRNA. (f) Immunoblot of control or PSAP siRNA-transfected HeLa cell media after treatment with (+) or without (−) BS³ crosslinker. Graphed values represent the mean±s.e.m. *Differs from 0 M guanidine-HCl treatment, P<0.05; **P<0.01; ***P<0.0001 by one-way ANOVA (a) or Student's t-test (b).