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. 2016 Jun 30;7:11988. doi: 10.1038/ncomms11988

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Mpath re-ordered single cells along the muscle and non-muscle developmental trajectories respectively. (a) Heatmap and clustering of genes that were differentially regulated during the development from 0 to 72 h (genes in rows and cells in columns). Cells were placed in the order of increased differentiation. Clustering of genes identified seven distinct groups. (b) Loess smoothed line chart visualization of dynamic trends of gene expression per group with respect to pseudo-temporal re-ordering. (c–h) Gene ontology analysis identified biological processes significantly (adjusted P value<0.05, DAVID gene ontology analysis) enriched by genes from group 1 (c), group 6 (d), group 5 (e), group 2 (f), group 3 (g) and group 4 (h).