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ASQ reaction
| Concentration | Volume (μl) | Final concentration | |
|---|---|---|---|
| DNA template | — | 1 | — |
| MyTaq reaction buffer (clear) | 5× | 2 | 1× |
| Allele-specific primers (WT + Mut) | 1 μM | 0.5 | 50 nM |
| Locus-specific reverse primer | 10 μM | 0.5 | 500 nM |
| Fluorescent probes + quenchers (FAM + HEX + FAM-Q + HEX-Q) | 50 μM | 0.1 | 500 nM |
| HS MyTaq DNA polymerase | 5 units/μl | 0.1 | 0.5 units |
| H2O | — | q.s. | — |
| Total | — | 10 | — |
