Figure 5. Lst4 is not an orthologue of FNIPs in yeast.

(a) BY4741 yeast strain expressing GAL1-LST4-GST was grown on either YPED (glucose) or YPGal (Galactose) media at 30 °C for 8 h. Endogenous yHsp90 immunoprecipitation (IP) and co-IP Lst4-GST was detected by immunoblotting. (b) In vitro ATPase activity of the yHsp90–His₆ with indicated amounts Lst4-GST. All the data represent mean±s.d. (c) PP30 strain expressing either yHsp90 or Hsp90α as the sole copies of Hsp90 were transformed by GAL1–FLAG–FNIP1 or GAL1–FLAG–FNIP2. Cells were spotted at 1:10 dilution series on YPED or YPGal media. Plates were incubated at 30 °C for 2 days. (d) Yeast strains in i were grown on YPGal liquid media for 12 h and the expression of FLAG–FNIP1 and FLAG–FNIP2 were examined by immunoblotting.