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. 2016 Jun 29;7:12037. doi: 10.1038/ncomms12037

Figure 8. Overexpression of FNIPs sensitize HEK293 cells to Hsp90 inhibitor.

Figure 8

(a) HEK293 cells were transiently transfected with HA–FNIP1 or empty vector (EV), (b) or HA–FNIP2 or empty vector (EV), and treated with indicated amounts of GB. (c) Induction of apoptotic markers in lysates from a and b were shown by immunoblotting using anti-cleaved caspase-3 and cleaved PARP antibodies. (d) FNIP1, FNIP2 or both were silenced by siRNA in HEK293 cells and then treated with indicated amounts of GB. Apoptotic markers were examined by immunoblotting using anti-cleaved caspase-3 and cleaved PARP antibodies.