FIG. 4.

Expression of C53S AQP8 confers stress resistance to HeLa cells. (A) Clonogenic assays comparing the ability of stable HaloAQP8 wt- or C53S-expressing HeLa cells to form clonal colonies before and after heat stress. Wells are the result of twofold serial dilutions to allow visualization of single-cell-derived colonies. Results are represented as percentage of surviving colonies. Average of three experiments ± SEM. (B). Measurement of colony area distribution in both AQP8 wt and C53S-expressing HeLa cells before and after the stress. Each dot represents a single colony and its area is displayed in mm². (C). Quantification of the clonogenic capacity of stable HaloAQP8 wt and C53S-expressing HeLa cells after 7 days from irradiation with different doses. Data are represented as percentage of surviving colonies with respect to the number of colonies grown in control conditions ± SEM. (D). Quantification of the clonogenic capacity of stable HaloAQP8 wt and C53S-expressing HeLa cells after 7 days from a 24 h-treatment with the indicated doses of arsenic trioxide. Data are represented as in (C). *p < 0.05; ***p < 0.001. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars