FIG. 6.

AQP8 mutation in C53S influences stress-induced p38 phosphorylation. (A) Apoptosis in stable HaloAQP8 wt and C53S-expressing HeLa cells was monitored by Annexin-V staining, both immediately after inducing stress with or without 2 mM NAC or after 24 h of culture at 37°C. Data are expressed in percentage of Annexin-V-positive cells ± SEM. (B). The image shows a representative Western blot analysis, showing changes in phosphorylation of p38 on whole HeLa cell extracts. Cells stably expressing HaloAQP8 wt or C53S mutant were cultured for 3 h at 37°C or 42°C with or without 2 mM NAC. The right panel shows the quantification of phospho-p38 signal normalized against its total levels. Data are represented as fold change of p38 phosphorylation under stress conditions with respect to the signal obtained with unstressed controls. Average of eight experiments ± SEM. *p < 0.05. n.s., non significant. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars