FIG. 3.

Representative 10× fluorescent images (top) with corresponding brightfield images showing position of cells in zebrafish head directly below. (A) Glioblastoma-injected zebrafish imaged at 3 DPF with Multi Wavelength Cell Scoring Mask (white) used to delineate and count individual cells. (B) Same xenograft as image (A) with added concentric circles (yellow) used to delineate regions for quantifying cell migration/invasion in 80 μm distances from the centroid of the cell mass (blue circle). (C) Mock-injected zebrafish imaged at 3 DPF. No fluorescent cell count data obtained from zebrafish mock injection, which consisted of cell medium only.