FIG. 6.

Quantitative analysis of human glioblastoma (U87MG) cell spread following LY294002 or vehicle control exposure. The number of migrated cells within concentric 80 μm distance regions from the centroid of U87MG mass was measured. Data reported as relative number of cells migrated within each distance region normalized, by division, to the total number of cells at 3 DPF. The 0 treatment contained 0.5% DMSO, which was maintained across all groups. The plus indicates the overall mean of each treatment group. These values were determined from three independent experiments (left, middle, and right panels, n = 13–32 per experiment). Note changes in y axis scale between the different distance regions. Significant differences for all pairwise comparisons between treatments are indicated by letters (A, B), p < 0.05. †Datasets for which Kruskal–Wallis one-way ANOVA was performed.