FIG. 3.

(A) Maximum mitochondrial respiration of NE-4C stem cells and differentiating NE-4C neurons was determined as the OCR of FCCP-treated cells in the percentage of OCR in starvation (100%). The columns represent averages and standard errors of means (n ≥ 8). (B) The proton leak of NE-4C stem cells, differentiating NE-4C neurons, primary neurons, and astrocytes was determined as the percentage of OCR remaining after blocking the mitochondrial ATP-synthase with oligomycin and subtracting the nonmitochondrial O₂ consumption. The columns represent averages and standard errors of means (n ≥ 23). (C) The relative expression (RQ) of mitochondrial transcription factor A (tfam) in the investigated cells (n = 3) is shown in relation to the tfam/hprt ratio (1.00) in NE-4C neurons. OCR, oxygen consumption rate.