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. 2016 Mar 24;5(2):16. doi: 10.3390/plants5020016

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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13-LOX gene expression in different 13-lox mutant backgrounds. LOX2 expression analysed in WT, in lox6A and in lox3B lox4A double mutants. LOX3 and LOX4 expression was analyzed in WT, lox2-1, and lox6A single mutants, and the lox2-1 lox6A double mutant. LOX6 expression was analyzed in lox2-1 and the lox3B lox4A double mutant. Leaves 8 (wounded) and 13 (distal) were snap-frozen before (unfilled bars) and 1 h after the wounding (filled bars). Data are from three (controls) and three to four (wounded) biological replicates (±SD). Letters (a, b, and c) refer to significant differences (ANOVA and t-test; p < 0.05).