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. 2016 Jul 4;6:28890. doi: 10.1038/srep28890

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Quick-view analysis of GC-active bulk population sequences. Cells were sorted for IgM (−) in the DT40 Cl18 (a) and DT40 ΔPC4 (b) clones and for IgM (−)/GFP (+) in the DT40 ΔPC4^recon (c) clone. Following 2 weeks bulk growth (~25 generations) of the sorted population, gDNA was recovered from each and the rearranged Igλ VJ segments were sequenced. The Quick-view graphical representation that we use clearly identifies insertions and deletions (green and red circles respectively), transitions and transversions (blue and gold diamonds respectively), and highlights the “+1 G” (grey circle) creating the frameshift.