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. 2016 Jul 4;6:29113. doi: 10.1038/srep29113

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(A) Fiji/ImageJ segmentation procedure: confocal microscopy pictures of the droplets are first slightly denoised with a median filter then pictures are scaled 10 times and we used an active contour (snakes) routine to record the (x, y) Cartesian coordinates of the droplet interface after a visual comparison with the experimental picture. (B) Matlab curvature computation: the pixelation of the contour coordinates is suppressed by the application of a low-pass FFT filter. The local analytical curvature of the droplets shape is computed from the filtered data. Pictures show a zoomed area of the droplet contour.