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. 2016 Jun 15;6(2):26. doi: 10.3390/bios6020026

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Results of the template removal via chemical extraction. Two different parameters, stage condition, and different solutions were tested. (a) and (b) shows the results for the tilt tray condition applied to Dish 10 and Dish 21; (c) and (d) shows the results for the stationary condition applied to Dish 18 and Dish 19. Chemical extraction results were not as expected; in fact, an increase in the fluorescence intensity measurements was observed. Therefore, several extraction parameters and different extraction methods were analyzed. Please note that, in figures (c) and (d), the pre-extraction data were so low in comparison, they do not show on the graphs.