Figure 3.

(A–C) Results from GFAP and lectin stainings in the spinal cord (grey and white matter) of EAE-induced animals at 20 dpi (for both females and males Ast-IL6 KO and floxed mice) and 46 dpi (females). Number of mice per group as indicated. GFAP overall immunostaining (A) and the number of lectin-positive microglia (B) and vessels (C) are shown. ^★ p < 0.05 vs. floxed mice. (D) Representative GFAP at 100× (top) and lectin at 150× (bottom) stainings of 46 and 20 dpi females, respectively. Arrows indicate vessels. The discontinuous line separates grey matter (left) from white matter (right). All inserts are at 400×; at the top they, show astrocytic morphology in both grey and white matter, and at the bottom, they show a vessel.