Figure 2. Influenza virus induces a nuclear polarized redistribution of nucleolin.

A549 cells were infected with H3N2 (MOI 1) and fixed at 8 hpi. Immunofluorescence staining of NP (red) and nucleolin (green) were performed. Single optical sections through the top (A1), the midline (A2) and the bottom (A3) of nuclei (xy) and z-axis reconstruction (z1 and z2, respectively) are represented. Merged fluorescent signals are presented, as indicated. Scale bar = 10 μM. JAcoP plugin for imageJ was used to highlight the colocalization of the two stainings by thresholding the signal above the background⁸⁰. For easier visualization, colocalized pixels are colorized in white (right panels).