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. 2016 Jul 4;6:29006. doi: 10.1038/srep29006

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Copyright © 2016, Macmillan Publishers Limited

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Nucleolin is required for the association of vRNPs with the host chromatin during infection (A) A549 cells were infected with H3N2 virus for 24 h (MOI 1) or mock-infected. Fractionated lysates were analyzed by western blot for detection of nucleolin, NP, NEP, and subcellular markers β -tubulin, HMGB1 and histone H3. (B) A549 cells were transfected with either si-Nucleolin or si-Control. Two days after transfection, cells were infected with H3N2 for 24 hours (MOI 1). Cells were subfractionated and lysates were analyzed by western blot. H3 and β-tubulin were detected as loading and fractionation control. wh.: Whole fraction; cytoplasm: cytoplasmic fraction; nucleoplasm: nucleoplasmic fraction; ch150: low salt chromatin fraction; ch500: high salt chromatin fraction.