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. 2016 Feb 6;27(6):451–463. doi: 10.1089/hum.2015.172

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© Alvin C. Ma, et al., 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Figure 4. — Quality control of TALEN assembly, using colony PCR and restriction enzyme digestion. (A) Representative colony PCR using primers TAL_F1/TAL_R2. Correct colonies (+) result in characteristic laddering due to the repetitive nature of the incorporated RVDs. (B) Representative restriction enzyme digestion analyses with AatII/StuI are shown [these clones differ from those shown in (A)]. The size of the lower band is dependent on the number of TALE repeats within the TALEN, which can aid in determining correct clones. +, correct TALEN clones; *, incorrect TALEN clones.