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. 2016 Feb 6;27(6):451–463. doi: 10.1089/hum.2015.172

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© Alvin C. Ma, et al., 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Figure 5. — In vivo mutagenic activity of TALEN pairs assembled with the FusX system. (A) Comparison of somatic efficiency between TALEN pairs (with identical RVD arrays) assembled via the FusX system (FLT3 P1X, P2X, and P3X) or via the original GGT method (FLT3 P1, P2, and P3). Average results of 3 separate experiments analyzing groups of 10 embryos are shown. Error bars represent the SEM, and efficiencies of TALEN pairs were statistically analyzed with unpaired t tests. (B) Somatic efficiencies of 30 TALEN pairs assembled with the FusX system. Error bars represent the SEM. Detailed descriptions of these TALEN pairs are found in Table 4. (C) Design of CHD P1 (TALEN 13) targeting zebrafish chordin Exon-1. (D) Embryos injected with CHD P1 (panel iii) showed significant ICM expansion (arrowhead) at 24 hr postfertilization (hpf) compared with wild type (panel i), phenocopying the morpholino-mediated chordin knockdown (panel ii).