Table 2.

FusX system libraries required for constructing transcription activator-like effectors with 14.5, 15.5, and 16.5 repeats

Shown is detailed information including library names, plasmid quantities, description, and 5′ and 3′ overhang sequences (in boldface) produced by BsmBI digestion of the nine plasmid libraries required for making TALEs with 14.5 to 16.5 repeats. Compatible overhangs of destination vector with pFusX1 and pLR are boxed and underlined, respectively.

pLR, last half-repeat plasmid; RVD, repeat-variable diresidue; TALE, transcription activator-like effector; TALEN, TALE as nuclease.