Table 2.
Results of total broth ammonium mass balance. Reconciled concentrations provide the best estimates of the measurements obtained by least square minimization of the differences between the measurements and estimated amounts, weighted with respect to their measurement errors.
| Type of Sample | Concentration Measured 1 (µMbroth) | Recovery (%) | Reconciled Concentration * (µMbroth) |
|---|---|---|---|
| Intracellular BE (Aug 14) | 24.51 ± 2.15 | 124.50% | N.A. |
| Extracellular (Aug 14) | 6.23 ± 1.15 | ||
| Total broth BE (Aug 14) | 24.69 ± 5.25 | ||
| Intracellular BE5 (Oct 14) | 10.04 ± 1.93 | 77.93% | N.A. |
| Extracellular (Oct 14) | 1.05 ± 0.49 | ||
| Total broth BE5 (Oct 14) | 14.23 ± 0.23 | ||
| Intracellular CM5 (Oct 14) | 15.16 ± 1.24 | 96.66% | 15.57 ± 0.64 |
| Extracellular (Oct 14) | 1.05 ± 0.49 | 1.12 ± 0.46 | |
| Total broth CM5 (Oct 14) | 16.77 ± 0.57 | 16.68 ± 0.52 |
1 Average of three independent samples injected and quantified three times; N.A. = not applicable. BE: conventional boiling ethanol extraction; BE5: buffered boiling ethanol protocol.