Table 3.
Quantitative analysis of amino acid decay using different extraction methods. The results displayed are averages of three independent samples (in µmol/gCDW).
| 13C Addition | Extraction Solution | Intracellular Metabolite Concentration (µmol/gCDW) (% Recovery with Respect to 13C Addition after Quenching) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Ala | Glu | Asp | Gln | PyrGlu | Pyruvate | αKG | NH4+ | ||
| AQ | BE5 | 6.97 ± 0.21 | 52.79 ± 0.36 | 11.51 ± 0.14 | 10.77 ± 0.25 | 0.78 ± 0.02 | 1.96 ± 0.17 | 3.01 ± 0.05 | 3.46 ± 0.28 |
| BA | BE5 | 5.73 ± 0.25 (82.3%) 1 |
51.05 ± 0.75 (96.7%) |
10.80 ± 0.33 (93.9%) |
9.81 ± 0.19 (91.2%) |
1.11 ± 0.02 (141.9%) 1 |
N.M. | N.M. | N.M. |
| AQ | CM5 | 6.50 ± 0.07 | 51.95 ± 0.65 | 9.03 ± 0.09 2 | 10.85 ± 0.16 | 0.51 ± 0.02 2 | 0.21 ± 0.01 | 0.50 ± 0.01 | 2.29 ± 0.44 |
| BA | CM5 | 6.00 ± 0.05 (92.4%) 1 |
49.95 ± 0.30 (96.2%) |
8.90 ± 0.07 (98.5%) |
10.68 ± 0.07 (98.4%) |
0.57 ± 0.06 (112.2%) |
N.M. | N.M. | N.M. |
| AQ | BE | 7.86 ± 0.13 | 56.00 ± 0.95 | 11.54 ± 0.28 | 11.28 ± 0.14 | N.M. | 2.17 ± 0.24 | 2.81 ± 0.06 | 5.78 ± 0.20 |
gCDW = gram of cell dry weight. N.M. = not measured. In these cases, the analysis was not performed, therefore no experimental data are available; 1 Statistically significant difference found between AQ and BA samples, according to a t-test at a significance level of 0.05. 2 Statistically significant difference found between BE5 AQ and CM5 AQ samples, according to a t-test at a significance level of 0.05.