Figure 1. Mcp3 is a multicopy suppressor of mdm10Δ.

1. Rescue of growth phenotype. Wild‐type (WT) or mdm10Δ cells were transformed with the empty plasmid pYX142 (∅). In addition, mdm10Δ cells were transformed with pYX142 encoding MDM10 or MCP3. Cells were grown to an OD₆₀₀ of 1.0 and spotted on YPD or YPG plates in a 1:5 dilution series. Plates were incubated for growth at the indicated temperatures.
2. Over‐expression of Mcp3 partially rescues the mitochondrial morphology defect in mdm10Δ cells. Cells described in (A) expressing mitochondrially targeted GFP (mtGFP) were analysed by fluorescence microscopy. Typical images (fluorescence and bright field, BF) of the four different strains are shown (scale bar = 5 μm). The quantification shows the average percentage with standard deviation bars of three independent experiments (n = 3; SD; *P < 0.05 and as indicated; two‐tailed Student's t‐test) with at least 100 cells per experiment.