Figure EV2. Altered levels of Mcp3 have no effect on ERMES foci formation and colocalization with mitochondria.

• A, B
  WT and mcp3Δ cells (from BY4741 (A) or W303 (B) background) expressing mitochondrially targeted GFP (mtGFP) and RFP‐tagged Mmm1 were grown to mid‐logarithmic phase and then analysed by fluorescence microscopy. Representative images are shown. Scale Bars, 5 μm.
• C
  WT cells harbouring an empty plasmid or a plasmid overexpressing Mcp3 (BY4741 background) were transformed with plasmids encoding mitochondrially targeted GFP (mtGFP) and RFP‐tagged Mmm1 and were analysed as in (A). Scale Bar, 5 μm.