Figure EV4. Import of Mcp3 is dependent on the mitochondrial membrane potential.

Mitochondria isolated from wild‐type yeast cells were incubated with radiolabelled precursor protein Mcp3 for the indicated time periods (1, 5 or 15 min) in the absence or presence of CCCP or valinomycin. After import, mitochondria were reisolated and analysed by SDS–PAGE and autoradiography. Input, 20% of radiolabeled precursor applied in the reaction.