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. 2016 Jun 6;17(7):1044–1060. doi: 10.15252/embr.201541960

Figure 4. Quantification of the Ape1, Ams1, Atg19 protein abundances in living cells.

Figure 4

  1. Epifluorescence microscopy images of the prApe1‐GFP/Atg19‐mCherry/ypt7Δ, Ams1‐GFP/Atg19‐mCherry/ypt7Δ, Atg19‐GFP/Ape1‐mCherry/ypt7Δ Saccharomyces cerevisiae cells that were used to quantify the fluorescence intensity of GFP spots. Only colocalizing GFP and RFP spots were considered. BF represents bright‐field images.
  2. Bar plot showing the number of molecules of prApe1, Ams1, and Atg19 found in Cvt vesicles in living cells. Data are representative of two independent experiments in which a total of 217 prApe1‐GFP, 181 Ams1‐GFP, and 233 Atg19‐GFP spots were analyzed. Error bars indicate standard deviation (SD). ***P < 0.001; *P = 0.0176 (Z test).