Figure 4. Cell penetration of SPACE-EGF and SE-siRC.

In (a–d) cells were treated for 6 hours with PBS, SPACE, EGF, and SPACE-EGF, respectively. In (e–h) cells were incubated for 6 hours with siRNA, siRNA-SPACE, siRNA-EGF, and siRNA-SPACE-EGF, respectively. In (b) SPACE was also labeled fluorescently. In (i,j) the mean fluorescence intensities in melanoma cells after 6 hours were compared. Scale bar: 20 μm. The linked siRNA was GAPDH-siRNA. The experiments were repeated at least 5 times.