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. 2016 Jul 4;6:29159. doi: 10.1038/srep29159

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Copyright © 2016, Macmillan Publishers Limited

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B16 cells were incubated with 10 μM of c-Myc siRNAs, negative c-Myc SE-siRC and c-Myc SE-siRC for 48 hours, and then the apoptosis was assessed by the flow cytometry analysis. In this work, early apoptotic (Annexin V+/PI−), late apoptotic/necrotic (Annexin V+/PI+), and live (Annexin V−/PI−) populations were clearly identified.