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. 2016 Jun 10;8(6):55. doi: 10.3390/cancers8060055

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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RASSF1A promoter methylation percentage of individual CpGs sites in breast cancer patients, n = 73. The individual CpG methylation percentage indicates what percentage of DNA molecules is methylated at this site in the sample. Although the methylation pattern is different in different types of breast cancer, CpG2, 9, 13–14, 16, 19, 26 and 31 are the methylation hotspots in all types of breast cancer, suggesting a conserved methylation mechanism. IBC, inflammatory breast cancer; DCIS, ductal carcinoma in situ; TNBC, triple-negative breast cancer.