Figure 6.

RASSF1A expression and methylation in cell lines. (a,b) RASSF1A methylation results in its mRNA expression silencing in cell lines. GAPDH mRNA expression (a) was used to normalize RASSF1A expression, and similar results are obtained if using peptidylprolyl isomerase A as a reference gene (b). (c) p53 status and RASSF1A methylation in cell lines (n = 20 for all except for p53^Null cells, where n = 4). Cell lines were separated into groups according to their p53 status and average RASSF1A methylation plotted.