Table 1.

Primer sequences for the pyrosequencing and qRT-PCR.

Assay		Primer Sequence 5’-3’
Pyrosequencing
Assay 1 (20 CpGs)	Forward	GGATGTGGGGATTTTTTTTTTTTAGTATAG
	Reverse	Biotin-ACTTCAACCCCTCCCCCAAAA
	Sequencing	TTTTTTAGTATAGTAAAGTTGGT
Assay 2 (12 CpGs)	Forward	TTTCGGGTTTTATAGTTTTTGTATTTAGGT
	Reverse	Biotin-ACTCCCGCAACTCAATAAACTCAAACTC
	Sequencing	TTTAGTAGTTTAGTTTGGATTTTGG
MOAP1	Forward	GAGTGTTAGTTAGAGTTTAGGGGAGTTT
	Reverse	Biotin-CTCACCCCTCCCAACCCT
	Sequencing	AGGGGAGTTTGTTTT
qRT-PCR
GAPDH	Forward	CATGACAACTTTGGTATCGTG
	Reverse	GTGTCGCTGTTGAAGTCAGA
RASSF1a	Forward	CCTCTGTGGCGACTTCATCTG
	Reverse	CAACAGTCCAGGCAGACGAG
MOAP-1	Forward	CTCAATTGCTCCTCTCTGTACC
	Reverse	CATGACAACTTTGGTATCGTG
RASSF1c	Forward	GCTACTGCAGCCAAGAGGAC
	Reverse	AGGTGTCTCCCACTCCACAG
PPIA	Forward	GCCGAGGAAAACCGTGTACT
	Reverse	TGTCTGCAAACAGCTCAAAGG